Journal: Scientific Reports

Article Title: Development of a multiplex and sensitive lateral flow immunoassay for the diagnosis of periprosthetic joint infection

doi: 10.1038/s41598-019-52051-6

Figure Lengend Snippet: The protein levels of alpha-defensin and CRP in 10 synovial fluid samples analyzed by ELISA.

Article Snippet: Anti-alpha-defensin antibody, Human alpha-defensin 1 DuoSet ELISA and Human C-Reactive Protein/CRP DuoSet ELISA were obtained from R&D systems (Minneapolis, MN, USA), Goat anti-mouse immunoglobulin (IgG) antibody was supplied by Jackson ImmunoResearch Laboratories (West Grove, PA, USA).

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Scientific Reports

Article Title: Development of a multiplex and sensitive lateral flow immunoassay for the diagnosis of periprosthetic joint infection

doi: 10.1038/s41598-019-52051-6

Figure Lengend Snippet: Correlation between the signal intensities of msLFIA results and ELISA measurements of CRP and alpha-defensin in synovial fluid samples. The error bars represent the standard deviation of three independent experiments.

Article Snippet: Anti-alpha-defensin antibody, Human alpha-defensin 1 DuoSet ELISA and Human C-Reactive Protein/CRP DuoSet ELISA were obtained from R&D systems (Minneapolis, MN, USA), Goat anti-mouse immunoglobulin (IgG) antibody was supplied by Jackson ImmunoResearch Laboratories (West Grove, PA, USA).

Techniques: Enzyme-linked Immunosorbent Assay, Standard Deviation

Journal: Nutrients

Article Title: Synbiotic Supplementation Containing Whole Plant Sugar Cane Fibre and Probiotic Spores Potentiates Protective Synergistic Effects in Mouse Model of IBD

doi: 10.3390/nu11040818

Figure Lengend Snippet: Effect of B. coagulans spores, PSCF and synbiotic on immune markers in colon tissues and blood serum. Protein levels of cytokines including ( A ) IL-1α, ( B ) IL-1β, ( C ) IL-6, ( D ) IL-12, ( E ) TNF-α, ( F ) IFN-γ in proximal and distal colon explants as well as cytokine levels of ( G ) IL-1β, ( H ) IL-10, and ( I ) IL-12 in blood serum were analysed by Bio-plex. iNOS activity in colon tissues ( J ) measured by NOS activity assay and CRP levels in serum ( K ) by ELISA. Statistical significance among groups evaluated by one-way ANOVA followed by Tukey’s test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 vs. DSS-colitic group and data expressed as mean ± SEM ( n = 3 per group).

Article Snippet: The levels of C-reactive protein (CRP) in serum from respective groups ( n = 3 samples/group) were analysed using Mouse C-Reactive Protein/CRP Quantikine Elisa kit (MCRP00, R and D Systems, Australia) following the manufacturer’s instructions.

Techniques: Activity Assay, Nos Activity Assay, Enzyme-linked Immunosorbent Assay

Journal: International journal of molecular sciences

Article Title: Modulation of the Cardiovascular Risk in Type 1 Diabetic Rats by Endurance Training in Combination with the Prebiotic Xylooligosaccharide.

doi: 10.3390/ijms251810027

Figure Lengend Snippet: Figure 4. Study design. BP—blood pressure, HR—heart rate, MTE—maximum time to ex- haustion, AC—abdominal circumference, T-AOC—total antioxidant capacity, Il-6—interleukin-6, hsCRP—high-sensitive C-reactive protein, NADH + H+—nicotinamide adenine dinucleotide hydride, CoQ—coenzyme Q, LDH—lactate dehydrogenase, SDH—succinate dehydrogenase.

Article Snippet: Serum T-AOC, IL-6 and hsCRP were analyzed on an enzyme-linked immunosorbent assay (ELISA) microplate reader HumanReader.HS, HUMAN (Wiesbaden, Germany) via commercially available kits [Rat Total antioxidant capacity, T-AOC Elisa kit, Nanjing Pars Biochem CO., Ltd., Nanjing, China; Rat IL-6 (Interleukin 6) ELISA Kit, Elabscience Biotechnology Inc., Houston, TX, USA; Rat hsCRP (high-sensitivity, C-Reactive Protein) Elabscience Biotechnology Inc., Houston, TX, USA].

Techniques:

Journal: ImmunoTargets and Therapy

Article Title: Recombinant Antithrombin Alleviated Pulmonary Injury and Inflammation in LPS-Induced ARDS by Inhibiting IL17a/NF-κB Signaling

doi: 10.2147/ITT.S502925

Figure Lengend Snippet: rAT reduced pulmonary inflammation in LPS-induced ARDS model mice. ( A–D ) The levels of inflammatory factors, including IL-6 ( A ), TNF-α ( B ), IL-8 ( C ), and hs-CRP ( D ), were decreased in the serum of the rAT-treated group, as detected by ELISA. ( E ) Immunohistochemical staining for F4/80 revealed that rAT treatment reduced the proportion of macrophages in the lung tissue of LPS-induced ARDS model mice. ( F ) Immunohistochemical staining for MRCI, a marker of M2 macrophages, showed that rAT treatment increased the proportion of M2 macrophages in the lung tissue of LPS-induced ARDS model mice. ( G ) Immunohistochemical staining for Ly6G, a marker of neutrophils, showed that rAT treatment reduced the proportion of neutrophils in the lung tissue of LPS-induced ARDS model mice. All the data are presented as the means ± SDs of three independent experiments. One-way ANOVA, *p < 0.05, **p < 0.01, ***p < 0.001, ns, not significant; scale bar, 50 μm.

Article Snippet: A mouse CXCL15 ELISA Kit (E-EL-M0269) and a mouse hs-CRP ELISA Kit (E-EL-M0677) were purchased from Elabscience (Wuhan, China).

Techniques: Enzyme-linked Immunosorbent Assay, Immunohistochemical staining, Staining, Marker

Journal: ImmunoTargets and Therapy

Article Title: Recombinant Antithrombin Alleviated Pulmonary Injury and Inflammation in LPS-Induced ARDS by Inhibiting IL17a/NF-κB Signaling

doi: 10.2147/ITT.S502925

Figure Lengend Snippet: The efficacy of rAT in mitigating lung injury, suppressing the immune response, and inhibiting the activation of the NF-κB signaling pathway in LPS-induced ARDS mice were diminished by the administration of IL-17a. ( A ) ELISA results demonstrated that the administration of IL17a inhibited the ability of rAT to reduce inflammatory factors, including IL-6, TNF-α, and IL-8, in the serum of LPS-induced ARDS mice. ( B ) The analysis of the wet/dry weight ratio of the lung tissue revealed that the administration of IL17a counteracted the ability of rAT to alleviate pulmonary exudation in LPS-induced ARDS mice. ( C ) The administration of IL17a did not significantly affect the ability of rAT to reduce the number of cells in the BALF of LPS-induced ARDS mice. ( D ) The administration of IL17a attenuated the ability of rAT to reduce the concentrations of proteins in the BALF of LPS-induced ARDS mice. ( E ) Real-time PCR results showed that the administration of IL17a blocked the ability of rAT to downregulate the expression of target genes in the IL17a/NF-κB signaling pathway. ( F ) The protein levels of the NF-κB signaling pathway were assessed by Western blotting, and gray intensity analysis of the blots showed that the administration of IL17a in LPS-induced ARDS mice counteracted the ability of rAT to suppress the phosphorylation of IκBα, IKKα/β, and P65. The data are expressed as the means ± SDs (n=3 in each group). One-way ANOVA, *p < 0.05, **p < 0.01, ***p < 0.001, and ns not significant.

Article Snippet: A mouse CXCL15 ELISA Kit (E-EL-M0269) and a mouse hs-CRP ELISA Kit (E-EL-M0677) were purchased from Elabscience (Wuhan, China).

Techniques: Activation Assay, Enzyme-linked Immunosorbent Assay, Real-time Polymerase Chain Reaction, Expressing, Western Blot, Phospho-proteomics

Journal: PLoS ONE

Article Title: Aortic Calcification and Femoral Bone Density Are Independently Associated with Left Ventricular Mass in Patients with Chronic Kidney Disease

doi: 10.1371/journal.pone.0039241

Figure Lengend Snippet: Biochemical data for all subjects and according to presence or absence of abdominal aortic calcification.

Article Snippet: High sensitive C-reactive protein (hsCRP) was measured using a validated immunoassay (IBL International GMBH, Hamburg, Germany).

Techniques: